Setiyaningsih, Fera Yuli, Jayasa, Pande Made Dwi, Sujuti, Hidayat and Retnani, Diah Prabawati (2018) Pengaruh pemberian Extra Virgin Olive Oil terhadap jumlah Sel Granulosa dan kadar 17 β Estradiol tikus yang di papar Rhodamin B. Journal of Ners and Midwifery, 5 (3). pp. 241-248. ISSN 2548-3811
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Abstract: The use of dyes in food today is very apprehensive. Many foods contain dyes that are prohibited to be used in food. The addition of the dye will have an impact on health. The aim of this research was to know the effectiveness of the administration of extra virgin olive oil to the number of granulosa cell and 17β estradiol level of Rats exposed by rhodamin B. The design of this study was true experimental with post test approach of group design control. The subject of the study was 30 white mice Rattus novergicus in the age of 12 weeks weight average 220 grams and in healthy condition. Rats were divided into 5 groups. Control group without treatment, positive control treatment with rhodamine B 18 mg / 200gr. Treatment 1 administration of rhodamine B 18 mg / 200gr and EVOO (Extra Virgin Olive Oil) 1.5 ml / KgBB, treatment 2 rhodamine B 18 mg / 200gr and EVOO 3 ml / KgBB, treatment 3 rhodamine B 18 mg / 200gr and EVOO 4,5 ml / KgBB. The number of granulosa cells was determined by HE staining, the level of 17β estradiol with ELISA. The data was analyzed by Kruskal Wallis test, Spearman Rank and Simple Linear Regression with degree of significance α <0.05. The average number of granulosa cells and the lowest levels of 17β estradiol were found in the positive control group and the highest average was in the treatment group 3. There was one dose that could significantly increase granulosa cells and the beta estradiol levels compared with the treatment group the other with niali α <0.05. The administration of EVOO affected the number of granulosa cells and the levels of 17β estradiol in rats exposed by rhodamine B. Keyword: granulosa cells, estradiol, rhodamin B, oxidative stress
| Item Type: | Article |
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| Subjects: | ?? R1 ?? |
| Divisions: | ?? v5i3 ?? |
| Depositing User: | FRISCO TALISTI |
| Date Deposited: | 30 Jan 2019 07:56 |
| Last Modified: | 09 Dec 2019 07:32 |
| URI: | http://repository.phb.ac.id/id/eprint/368 |
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